Please do share with regards Age and illness details of patient in our email ( AsmiConsultancyHerbals@gmail.com ) so we can confirm the details on Medically recommended intake Dosage of Organic Fagonia cretica Tea. Because in an early case of Cancer, the mixing ratio with regards Organic Fagonia cretica is Leaf 40 : Flower 60 while in Stage 4 / Terminal illness, its Leaf 10 : Flower 90 and similarly as per illness severity / type of carcinoma, the mixing ratio of Leaf : Flower and daily intake dosage also varies.

We have Registered TradeMark of Organic Fagonia cretica Tea so all patients can be absolutely assured with regards the Authenticity of our Product and the accountability of our Herbal Formulations for cancer patients.

Using flow cytometric analysis of cells labeled with cyclin A, annexin V and propidium iodide, we describe a time and dose-dependent arrest of the cell cycle in G0/G1 phase of the cell cycle and apoptosis following extract treatment in MCF-7 (WT-p53) and MDA-MB-231 (mutant-p53) human cancer cell lines with a markedly reduced effect on primary human mammary epithelial cells. Analysis of p53 protein expression and of its downstream transcription targets, p21 and BAX, revealed a p53 associated growth arrest within 5 hours of extract treatment and apoptosis within 24 hours. DNA double strand breaks measured as γ-H2AX were detected early in both MCF-7 and MDA-MB-231 cells. However, loss of cell viability was only partly due to a p53-driven response; as MDA-MB-231 and p53-knockdown MCF-7 cells both underwent cell cycle arrest and death following extract treatment. p53-independent growth arrest and cytotoxicity following DNA damage has been previously ascribed to FOXO3a expression. Here, in MCF-7 and MDA-MB-231 cells, FOXO3a expression was increased significantly within 3 hours of extract treatment and FOXO3 siRNA reduced the extract-induced loss of cell viability in both cell lines.

Our results demonstrate for the first time that an aqueous extract of Fagonia (cretica) indica can induce cell cycle arrest and apoptosis via p53-dependent and independent mechanisms, with activation of the DNA damage response. We also show that FOXO3a is required for activity in the absence of p53. Our findings indicate that Fagonia (cretica) indica aqueous extract contains potential anti-cancer agents acting either singly or in combination against cancer cell proliferation via DNA damage-induced FOXO3a and p53 expression.